The Twenty First Annual Clinical Course on Flow and Image Cytometry
The Twenty First Annual Clinical Course on Flow and Image Cytometry will be held this year at the Roswell Park Cancer Institute in Buffalo, New York, USA. As in past years, the Course will include hands-on laboratory and computer work in small groups led by expert faculty, lectures on a variety of general and advanced topics, and many opportunities for informal discussion. The Course will emphasize basic flow theory, current best practices in the clinical laboratory, flow cytometric diagnosis of leukemia and lymphoma, and frontiers for the future. The Course will be limited to 45 participants who will rotate in small groups through an individualized selection of seven laboratory modules. The cause is directed at individuals with some experience in flow cytometry
Participants will gain an understanding of the principles of flow cytometry and up-to-date knowledge of the ways in which cytometry can be applied to, among others, the following clinical problems:
- leukemia and lymphoma phenotyping and interpretation of histograms;
- analysis of myeloid disorders & granulocyte function;
- analysis of intracellular epitopes and signaling pathways in leukemia;
- staining and histogram analysis for DNA cell cycle stage and ploidy;
- high level multicolor clinical flow cytometry;
- quality control, standardization and proper experimental design;
- flow cytometric diagnosis of primary immunodeficiencies;
- reference and alternative methods for CD4 and CD34 measurements;
- selection of fluorochromes and compensation issues;
- staining of leukocytes with tetramers to evaluate antigen specificity;
- use of tracking dyes to follow cell proliferation;
- imaging techniques for chemosensitivity and FISH analysis.
Roswell Park Cancer Institute is the Nation’s first comprehensive cancer center and is located within the Buffalo – Niagara Medical Campus in Buffalo, New York. Centrally located in the ‘City of Good Neighbors’, we are 10 minutes from the Albright Knox Art Gallery, Delaware Park, the Buffalo Zoo and Erie Basin Marina. Our campus is situated adjacent to the Allentown District, a community noted for its artists, antiques, gourmet restaurants and coffeehouses. Be sure to make the short walk to the world-famous Anchor Bar; the restaurant where the Buffalo chicken wing was created. Lectures and laboratories will be held at the Medical Center. Accommodations and social events will make use of the Institute’s facilities and will include a trip to Niagara Falls. Transportation for events will be provided.
FACULTY
Roswell Park Cancer Institute
- MYRON S. CZUCZMAN, MD
Chief, Lymphoma Section, Department of Medicine - GEORGE DEEB, MD
Depts. of Pathology, Associate Director, Department of Flow and Image Cytometry - HANS MINDERMAN, PhD
Dept. of Medicine, Co-Director, Department of Flow and Image Cytometry - CARLETON STEWART, PhD
Department of Pathology, Emeritus Director, Department of Flow and Image Cytometry - PAUL K. WALLACE, PhD §
Dept. of Pathology, Director, Department of Flow and Image Cytometry
Guest Faculty Includes
- SAMER Z. AL-QURAN, M.D.
Depts of Pathology, Immunology and Laboratory Medicine
University of Florida College of Medicine, Gainesville, Florida - ALBERT D. DONNENBERG, PhD
Depts. of Medicine & Infectious Diseases and Microbiology
University of Pittsburg Medical Center, Pittsburg, Pennsylvania
ALICE GIVAN, PhD - Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center
Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire - MARC G. GOLIGHTLY, PhD, SI (ASCP)
Clinical Immunology and Flow Cytometry Laboratories
State University of New York, Stony Brook, New York - JOHN MARTIN, PhD
Bioscience Division
Los Alamos National Laboratory, Los Alamos, New Mexico - KATHARINE MUIRHEAD, PhD
SciGro, Inc., Madison, Wisconsin - MAURICE R.G. O’GORMAN, Ph.D, D(ABMLI), MBA
Depts. Of Pediatrics and Pathologyand Laboratory Medicine
Northwestern University Medical School, Chicago, Illinois - T. VINCENT SHANKEY, PhD
Beckman Coulter Adv. Technology/Cell Analysis Group
Miami, Florida - HOWARD SHAPIRO, MD
The Center for Microbial Cytometry, West Newton, Massachusetts - ALAN WAGGONER, PhD
Carnegie Mellon University, Pittsburgh, Pennsylvania - BRENT L. WOOD, MD, PhD
University of Washington, Director Hematopathology Laboratory
Seattle, Washington
§ course director
PROGRAM INFORMATION
Registration for the Course will begin at 9:00 AM on Sunday, August 30th. The program will begin at 10:00 am with a series of lectures covering the principles and practice of cytometric analysis. This all-day session will be followed by an informal reception and dinner. The schedule for the rest of the week will include morning and afternoon lectures on specific topics each followed by 3-4 hour blocks of hands-on laboratory and computer work. Participants will rotate through each of the seven practical modules and have a choice of selecting laboratory tracks focusing on either Flow Cytometric Clinical Sciences or Interpretation Leukemia and Lymphoma Flow Cytometric data. Evenings will provide time for question periods, panel discussions, and informal interactions as well as a keynote address and workshop by Dr. Alan Waggoner.
Tuesday afternoon will be free for touring Buffalo, hiking, swimming, boating, or just relaxing. On Thursday evening, there will be a celebratory closing dinner at the Top of the Falls Restaurant, in Niagara Falls, USA, located just steps from the magnificent Niagara Falls where you will enjoy a spectacular view of the Illumination of the Falls. The Course will finish on Friday morning, September 4th, with a lecture by Dr. Howard Shapiro.
DATES/TIME: The Course begins at 10:00 a.m., Sunday, August 30th, and concludes at about noon, Friday, September 4th.
PLACE: Lectures and laboratories will take place at Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo, New York, USA.
AUDIENCE: The Course is directed at individuals with some experience in flow cytometry. The Course is limited to approximately 45 participants.
ACCOMMODATIONS: A block of rooms has been reserved at the Comfort Suites Hotel, 601 Main Street, Buffalo, NY at a special rate of $95.00 per night, single/double. Complimentary full buffet breakfast is included in this rate. For reservations, please call (716) 854-5500. The Comfort Suites is located just 5 minutes from RPCI, and within walking distance to Shea’s Performing Arts Center and many restaurants and nightclubs. Shuttle service is available to and from the hotel and RPCI.
Please reserve your hotel room no later than August 14th, 2009 to ensure this special rate. When making your hotel reservation, please notify the hotel that you will be attending the Flow & Image Cytometry Course at Roswell Park Cancer Institute. Other lodging is available in the Buffalo area; information will be sent on request.
ACCREDITATION: Roswell Park Cancer Institute is accredited by the Accreditation Council for Continuing Medical Education to sponsor continuing medical education for physicians. Roswell Park Cancer Institute designates this educational activity for a maximum of 40 AMA PRA Category 1 Credits TM. Physicians should only claim credit commensurate with the extent of their participation in the activity.
TRAVEL: Roswell Park Cancer Institute is served by Buffalo/Niagara International Airport which is approximately six miles from RPCI. Most of the major US and a number of the discount air carriers fly into Buffalo. The Comfort Suites offer shuttle transportation from the airport to the Hotel. Alternately, the Buffalo bus terminal is 2 miles away and the Amtrak rail station in Depew, NY is 11 miles from Roswell. Paid parking is available around the Cancer Center.
COURSE REGISTRATION FEE: $1,200 US Funds (increasing to $1,400 US Funds after August 1). The fee includes extensive course materials, refreshment breaks, lunches, and dinners (including reception and closing celebration).
CANCELLATION POLICY: There is a $25.00 administrative fee on all cancellations made before August 1st. Those canceling after August 1st will be charged half of the registration fee. No refunds will be given after August 21, 2009.
FURTHER INFORMATION: For registration, accommodation, and travel information or if you have any special needs, contact the CME Office at Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263 - telephone (716) 845-5706 or by e-mail to Denise Hammell (Denise.Hammell@RoswellPark.org). For course content information, contact by e-mail Paul Wallace (clinclourse@rpciflow.org) or by FAX at (716) 845-8806.
Registration: may be done on-line at: www.roswellpark.org/register

