Calibur w/FL-4 & loader options (2 laser cytometer)

Cell Quest Software (Macintosh OS)

Laser Excitation Sources: 488nm & 635nm

Detection:
FL-1 530/30 nm (FITC)
FL-2 585/42 nm (PE/PI)
FL-3 Long Pass 670 nm (PerCp)
FL-4 661/16 nm (APC)
SSC 488/10
FSC 488/10

FACScan 1 Roswell modified w/additional 4th (FL-4) detector

                                                                     (single 488nm laser)

Cell Quest Pro Software (Macintosh OS)

                         Laser Excitation Source: 488nm

Additional hardware compensation circuits have been added for FL-4:

FL4-%FL3, FL2-%FL4, FL3-%FL4, FL1-%FL4, FL4-%FL2,FL4-%FL1

Also a forgotten compensation network for FL3-%FL1

Detection:
FL-1 530/30 nm (FITC) (non-removable)
FL-2 575/26 nm (PE/PI) (removable)
FL-3 Long Pass 650 nm (PerCp) (removable)
FL-4 630/22 (Texas Red or Auto Flourscence Reduction) (removable)
SSC shorter than 560nm scattered light
FSC sees everything, no optical filter

FACScan 2 (Stock, no modifications, single laser)

Cell Quest Pro software (Macintosh OS)

Laser Excitation Source: 488nm (only light source)

Detection:
FL-1 530/30 nm (FITC)
FL-2 585/42 nm (PE/PI)
FL-3 Long Pass 650 nm (PerCp)
SSC shorter than 560nm scattered light
FSC sees everything, no optical filter

FACS Aria (3 laser sorting cytometer)
DiVa software (PC OS)
(all optical filters are removable)

Laser Excitation 488nm (blue laser):

Detection:
A- 780/60 PE-Cy7
B- 695/40 PerCP-Cy5.5 or PI
C- 610/20 PE-Texas Red
D- 575/26 PE or PI
E- 530/30 FITC
F- 488/10 SSC

Forward Scatter- 488/10

Laser Exitation 633nm (red laser)

Detection:
A- 780/60 APC-Cy7 (Atl. for Alexa 700: 730/30)
B- 660/20 APC

Laser Excitation 407nm (violet laser)

Detection:
A- 530/30 Alexa Flour 430
B- 450/40 Cascade blue, Pacific blue, Hoechst, Alexa Fluor 400

FACS Canto (2 laser cytometer)
DiVa software (PC OS)
(all optical filters are removable)

Laser Excitation 488nm (blue laser)

Detection:
A- 780/60 PE-Cy7
B- 670LP PerCP-Cy5.5 or PerCP
C- 585/42 PE
D- 530/30 FITC
E- 488/10 SSC
F- BLANK

G-BLANK

H-BLANK

Forward Scatter- 488/10

Laser Exitation 633nm (red laser)

Detection:
A- 780/60 APC-Cy7 (Atl. for Alexa 700: 730/30)
B- 660/20 APC

Vantage Cell Sorter with DiVa upgrade

                           (4 way sorting Flow Cytometer)

DiVa software (PC OS)

(all optical filters are removable)

                                 Laser Excitation 488nm (blue laser)

FL1- 530/30 FITC
FL2- 575/26 PE
FL3- 670/14 PE Cy5

                              Laser Excitation 350nm (100mw Ultraviolet, UV)

FL4- 424/44 Indo-1 (ca++) or Hoechst Side population (Stem Cells)
FL5- 650LP Hoechst Side population (Stem Cells)

Infrared Flow Cytometer

(Roswell modified FACScan, single laser)

Cell Quest Software (Macintosh OS)

(all optical filters are removable)

Laser Excitation 785nm

(Solid State, 40mw max)

Forward Scatter

Side Scatter

FL1- 820LP (Rockland IR-800, PTIR membrane dyes, Alexa 750,  

                                                                                      Indocyanine Green)

Detection features APD technology, extends detection wavelength >1000nm.